Temporal dynamics of CD69 expression on lymphoid cells

摘要: Lymphocyte activation remains an area of intense interest to immunologists and cell biologists the dynamics expression surface molecules during process are widely studied. The CD69 C-type lectin is reportedly earliest antigen on lymphocytes can be detected within hours mitogenic stimulation. Recently reports have described differential with respect different antigenic or stimuli. This study has investigated over time after mitogenic, allogeneic, cytokine target mediated T-cell NK subsets. It apparent that differ type method Mitogenic stimulation resulted in most rapid both T- cells while alloantigen induced a far slower response. Target gave paradoxical results + ve subset increased as proportion total but did not increase number. was due selective binding - their subsequent loss from lymphoid gate. We confirmed this by showing do lyse K562 cells. observation demonstrates caution needed analysis flow cytometric data based solely upon relative proportions discrete