Molecular differentiation of the four most commonly occurring Trichogramma (Hymenoptera: Trichogrammatidae) species in China
作者： Zheng-Xi LI
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摘要: Trichogramma dendrolimi, T. ostriniae, confusum and evanescens are the four most commonly occurring Tricho- gramma species with overlapping distribution in China. They frequently used egg parasitoids for biological control of lepidopterous crop pests It is difficult to differentiate because their small size lack differ- ences morphological characters. Different molecular markers were employed molecularly characterize these species, including direct amplification internally transcribed spacer 2 (ITS2) ribosomal DNA by polymerase chain reaction (PCR), restriction fragment length polymorphism (RFLP), random amplified polymorphic (RAPD) species-specific prim- ers. The results showed that ITS2 could not clearly discriminate but they be differentiated using RFLP pattern obtained endonucleases EcoRI HindIII. banding produced RAPD irreproducible so it a suitable way identify species. Finally, primers designed based on sequences unequivocally distinguish primer-based protocol proved convenient time saving method identification creating unique PCR product, which can sur- veying natural populations This first report prompt
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