Testing the usage of ITS2 and 28S-D2 markers in identifying some species of Ammophila, Prionyx, and Sphex genera (Hymenoptera: Sphecidae)

摘要: In addition to traditional methods, investigation of rDNA gene regions with molecular techniques is widely used identify insect taxa. this study, the usability primers (TrITS2, 28S-D2) belonging second expansion segment (D2) 28S subunit (28S-D2) and internal transcribed spacer (ITS2) were tested differentiate six species Sphecidae family namely Ammophila sabulosa, heydeni, Prionyx kirbyi, nudatus, Sphex flavipennis, funerarius for first time. DNA isolated from samples amplified by Polymerase Chain Reaction (PCR) digested restriction enzymes. PCR products enzyme analysis ITS2 obtained displayed species-specific bands indicating that these could be identified using TrITS2 However, neither nor sequence 28S-D2rDNA provided data. Consequently, marker was suitable only one genera (Prionyx), whereas 28S-D2 none taxa examined