Glucose metabolism and bioreduction of 2-butanone byCandida utilis studied by means of ion-exchange chromatography
作者： Cheanyeh Cheng , Jih-Hong Ma
DOI: 10.1016/S0021-9673(96)00890-4
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摘要: Abstract The metabolism of glucose during cell growth was important for the production yield yeast mediated bioreduction ketones. Thus, choice very simple acyclic ketone 2-butatone as substrate forCandida utilis can easily explain effect on enantioselective mechanism ketone. extended by adding sorbitol (polyol) pathway to Embden-Meyerhoff due existence a NADP-specific polyol dehydrogenase inC. utilis. metabolite, glycerol, produced via which could suppress ethanol pathway. reaction showed that ethanol, an energy source bioreduction, must be oxidized acetic acid activate oxidoreductase. formation acid, therefore, serve mark success bioreduction. On other hand, unconsumed and less added 2-butanone indicated negative Overall, microbial biotransformation toS-form 2-butanol studied ion-exchange chromatography successful.
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