Ovule rescue efficiency of Gossypium hirsutum × G. arboreum progeny from field-grown fruit is affected by media composition and antimicrobial compounds

摘要: Upland cotton (Gossypium hirsutum L.) is reproductively isolated from G. arboreum L. via post-zygotic breeding barriers. Literature on somatic embryogenesis of suggests a number media modifications that might also prove useful for ovule rescue interspecific crosses. Additionally, endogenous microbes are common in field grown and these potential contaminants must be controlled if progeny to obtained large-scale crossing followed by or embryo culture. This study compared nine tissue culture two antimicrobial overlay treatments factorial design. The were: 2 ml 250 mg l−1 cefotaxime, 50 tetracycline HCl, 2.5 amphotericin B, benomyl applied when the ovules were plated, no overlay. All contained cefotaxime. Crosses made at Stoneville, MS between upland cultivar DeltaPine 90 accession A2-190. Antimicrobial compounds greatly improved efficiency obtaining progenies field-grown fruit. Germination was not affected nor did treatment interact with media. Media significantly germination. Of studied, highest frequency germination observed MSB 1.9 g additional KNO3. addition 0.5 asparagine 1 glutamine affect seedlings obtained. A filter paper growing surface NAA 0.05 kinetin disadvantageous.