Redox-Sensitive PEG-Polypeptide Nanoporous Particles for Survivin Silencing in Prostate Cancer Cells
作者： Francesca Cavalieri , Giovanni L. Beretta , Jiwei Cui , Julia A. Braunger , Yan Yan
DOI: 10.1021/ACS.BIOMAC.5B00562
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摘要: We report the engineering of intracellular redox-responsive nanoporous poly(ethylene glycol)–poly(l-lysine) particles (NPEG–PLLs). The obtained exhibit no toxicity while maintaining capability to deliver a small interfering RNA sequence (siRNA) targeting anti-apoptotic factor, survivin, in prostate cancer cells. redox-mediated cleavage disulfide bonds stabilizing NPEG–PLL–siRNA complex results release bioactive siRNA into cytosol PC-3 cells, which, turn, leads effective silencing (∼59 ± 8%) target gene. These findings, under optimal conditions, indicate that NPEG–PLLs may protect therapeutic nucleic acid extracellular and environments, thus preventing occurrence competitive interactions with serum cytosolic proteins as well degradation by RNase. trafficking final fate were investigated combination deconvolution microscopy, fluores...
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