Pyrosequencing cut-off value identifying BRAFV600E mutation in fine needle aspiration samples of thyroid nodules.

摘要: CONTEXT Recently, tremendous efforts have been made towards the development of sensitive techniques to detect BRAF(V600E) mutation in fine needle aspiration biopsy (FNAB) samples. However, newly developed quantitative and semi-quantitative methods, such as dual-priming oligonucleotide (DPO)-based multiplex polymerase chain reaction (PCR), potential generate false-positive (FP) results. OBJECTIVES To eliminate possibility FP results, we generated a receiver operating characteristic (ROC) curve investigate diagnostic accuracy pyrosequencing using data. DESIGN Cytological diagnoses 983 thyroid nodules were according Bethesda System 2007. The was analysed by pyrosequencing, statistical analyses performed. RESULTS Of nodules, 902 adopted evaluate value pyrosequencing. number pathologically confirmed malignancies 192, which 182 papillary cancer (PTC). By generating an ROC curve, defined optimal cut-off mutant allele peak 5·95% (area under 0·849; sensitivity, 0·55; 1-specificity, 0). When applied this selective value, PTCs positive for 99 (54·4% total PTCs). With cytology alone, sensitivity specificity detecting malignancy 71·2% 100%, respectively. Pyrosequencing improved from 78·5% (McNemar's test, P 0·05). CONCLUSIONS is effective method FNAB allowing be determined, improves while eliminating