Detection of Reactive Oxygen Species via Endogenous Oxidative Pentose Phosphate Cycle Activity in Response to Oxygen Concentration IMPLICATIONS FOR THE MECHANISM OF HIF-1α STABILIZATION UNDER MODERATE HYPOXIA
作者： Stephen W. Tuttle , Amit Maity , Patricia R. Oprysko , Alexander V. Kachur , Iraimoudi S. Ayene
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摘要: The oxidative pentose phosphate cycle (OPPC) is necessary to maintain cellular reducing capacity during periods of increased stress. Metabolic flux through the OPPC increases stoichiometrically in response a broad range chemical oxidants, including those that generate reactive oxygen species (ROS). Here we show sensitivity sufficient detect low levels ROS produced metabolically as function percentage O2. We observe significant decrease activity cells incubated under severe and moderate hypoxia (ranging from
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