Endothelial Cell Loss after Descemet Stripping with Endothelial Keratoplasty: Influencing Factors and 2-Year Trend

摘要: Purpose To identify factors correlated with early endothelial cell loss after Descemet stripping keratoplasty (DSEK) and to document over a 2-year period. Design Cross-sectional longitudinal retrospective analyses. Participants A series of 263 eyes in 216 patients who were treated DSEK examined at the same center surgery. Methods Six-month density (ECD) measurements analyzed by multivariate analysis variance preoperative, operative, postoperative that significantly influenced loss. In addition, ECD compared longitudinally 6, 12, 24 months grafting. Main Outcome Measures Central transplantation. Results Mean was 2000±550 cells/mm 2 6 DSEK, representing 34±18%. Factors associated lower 6-month use single-point fixation forceps compressed donor tissue only tip during graft insertion ( P = 0.025) absence any secondary reattachment procedure 0.035). also less when performed as combined procedure; cases, inserted through clear corneal incision, whereas standard it scleral tunnel which likely exerted more compression. Cell comparable manual microkeratome dissection techniques 0.91). Postoperative positively 0.45), death-to-use time (range, 2–8 days; 0.86), recipient demographics. 34 longitudinally, mean 3100±250 before surgery, 2000±540 months, 1900±480 1 year, 1800±490 years. Conclusions year higher than recent penetrating center, consistent manipulation DSEK. reduced certain variations surgical technique.