Shikonin causes apoptosis by up-regulating p73 and down-regulating ICBP90 in human cancer cells.
作者： Soon Young Jang , Darong Hong , Seo Young Jeong , Jong-Ho Kim
DOI: 10.1016/J.BBRC.2015.07.131
关键词:
摘要: Shikonin, a natural naphthoquinone isolated from the Chinese traditional medicine Zi Cao (purple gromwell), is known to suppress growth of several cancer cell types. In this study, we evaluated pro-apoptotic effects shikonin on MCF-7 and HeLa cells, investigated underlying mechanism. Shikonin-induced apoptosis was associated with activation caspase-3, poly(ADP-ribose) polymerase (PARP) cleavage, up-regulation p73, down-regulation BCL-2. Shikonin also induced tumor suppressor gene, p16INK4A. Increasing transcriptional activity p16INK4A by treatment, observed in luciferase promoter assay, reflects reduced binding ICBP90 (inverted CCAAT box protein, 90 kDa), which are involved its partner, DNMT1 (DNA methyltransferase 1). On basis these results, conclude that causes via p73-related, caspase-3–dependent pathway.
sci-hub.se 参考文章(35)
Masaharu Takigawa, Kenji Takada, Yusuke Kimura, Fujio Suzuki, Tomoyuki Hisa, Shikonin, an ingredient of Lithospermum erythrorhizon, inhibits angiogenesis in vivo and in vitro. Anticancer Research. ,vol. 18, pp. 783- 790 ,(1998)
Jean-Marc Jeltsch, Christian Bronner, Pierre Oudet, Yves Lutz, Raphaël Hopfner, Cristi Marin, Jean-Pierre Bellocq, Marc Mousli, Angélique Voulgaris, ICBP90, a Novel Human CCAAT Binding Protein, Involved in the Regulation of Topoisomerase IIα Expression Cancer Research. ,vol. 60, pp. 121- 128 ,(2000)
M. Levrero, V. De Laurenzi, A Costanzo, S Sabatini, J Gong, JYJ Wang, G Melino, The p53/p63/p73 family of transcription factors: overlapping and distinct functions Journal of Cell Science. ,vol. 113, pp. 1661- 1670 ,(2000) , 10.1242/JCS.113.10.1661
William Cho, Nasopharyngeal carcinoma: molecular biomarker discovery and progress Molecular Cancer. ,vol. 6, pp. 1- 9 ,(2007) , 10.1186/1476-4598-6-1
George V. Avvakumov, John R. Walker, Sheng Xue, Yanjun Li, Shili Duan, Christian Bronner, Cheryl H. Arrowsmith, Sirano Dhe-Paganon, Structural basis for recognition of hemi-methylated DNA by the SRA domain of human UHRF1 Nature. ,vol. 455, pp. 822- 825 ,(2008) , 10.1038/NATURE07273
Mourad Kaghad, Helene Bonnet, Annie Yang, Laurent Creancier, Jean-Christophe Biscan, Alexandre Valent, Adrian Minty, Pascale Chalon, Jean-Michel Lelias, Xavier Dumont, Pascual Ferrara, Frank McKeon, Daniel Caput, Monoallelically expressed gene related to p53 at 1p36, a region frequently deleted in neuroblastoma and other human cancers. Cell. ,vol. 90, pp. 809- 819 ,(1997) , 10.1016/S0092-8674(00)80540-1
Michaël Jeanblanc, Marc Mousli, Raphaël Hopfner, Kawtar Bathami, Nadine Martinet, Abdul-Qader Abbady, Jean-Claude Siffert, Eric Mathieu, Christian D Muller, Christian Bronner, The retinoblastoma gene and its product are targeted by ICBP90: a key mechanism in the G1/S transition during the cell cycle Oncogene. ,vol. 24, pp. 7337- 7345 ,(2005) , 10.1038/SJ.ONC.1208878
Yue Hou, Xiuli Bi, Jinghua Xu, Xia Liu, Xichun Xia, Ning Li, Shikonin induces apoptosis in the human gastric cancer cells HGC-27 through mitochondria-mediated pathway Pharmacognosy Magazine. ,vol. 11, pp. 250- 256 ,(2015) , 10.4103/0973-1296.153074
Mahmoud Alhosin, Abdurazzag Abusnina, Mayada Achour, Tanveer Sharif, Christian Muller, Jean Peluso, Thierry Chataigneau, Claire Lugnier, Valérie B. Schini-Kerth, Christian Bronner, Guy Fuhrmann, Induction of apoptosis by thymoquinone in lymphoblastic leukemia Jurkat cells is mediated by a p73-dependent pathway which targets the epigenetic integrator UHRF1. Biochemical Pharmacology. ,vol. 79, pp. 1251- 1260 ,(2010) , 10.1016/J.BCP.2009.12.015
Abdurazzag Abusnina, Mahmoud Alhosin, Thérèse Keravis, Christian D. Muller, Guy Fuhrmann, Christian Bronner, Claire Lugnier, Down-regulation of cyclic nucleotide phosphodiesterase PDE1A is the key event of p73 and UHRF1 deregulation in thymoquinone-induced acute lymphoblastic leukemia cell apoptosis Cellular Signalling. ,vol. 23, pp. 152- 160 ,(2011) , 10.1016/J.CELLSIG.2010.08.015