Role of protein kinase C in the acrosome reaction of mammalian spermatozoa.

摘要: Mammalian spermatozoa undergo a Ca(2+)-dependent exocytotic event before fertilization which is known as the acrosome reaction. The process of exocytosis in several cell systems mediated by protein kinase C (PKC)-catalysed phosphorylation. Addition phorbol 12-myristate-13-acetate or membrane-permeant diacylglycerol analogue 1-oleoyl-2-acetylglycerol, are potent activators PKC, to bovine resulted stimulation This was inhibited low concentrations (50% inhibition at 0.7 nM) PKC inhibitor staurosporine. specific activity extremely comparison with other cells; however, it comparable found human spermatozoa. Immunohistochemical analysis using anti-PKC antibodies revealed staining equatorial segment, post-acrosomal region and upper head. We propose that involved mammalian sperm