Transplanted xenogenic bone marrow stem cells survive and generate new bone formation in the posterolateral lumbar spine of non-immunosuppressed rabbits

摘要: Bone marrow stem cells (BMSCs) are pluripotent that have been used to facilitate bone repair because of their capability differentiating into osteoblasts. However, it is well known the number BMSCs with osteogenic potential decreases in patients old age, osteoporosis, and metabolic diseases. In such conditions, xenogenic may provide an alternative autologous BMSCs. current study, we investigated transplanted survive generate new formation posterolateral lumbar spine non-immunosuppressed rabbits. The were obtained from bilateral femurs four male rats, cultured expanded medium osteoinduction supplement. (1,000,000 cells) rats loaded onto 5 cc compression resistant matrix (CRM; Medtronic Sofamor Danek, USA) implanted bilaterally L4-5 intertransverse processes 16 female rabbits (xenogenic + CRM group). received alone as controls (CRM To exclude possibility migration transverse recipient rabbits, did not decorticate processes. No any immunosuppressive medications during experiment. Four each both experimental control groups killed at 1, 2, 4, 6 months postimplantation, underwent radiological histological analyses for evaluation formation. polymerase chain reaction (PCR) Sry gene (Y-chromosome-specific marker) was evaluate survival expression clearly identified spines all group 1–6 postimplantation. Serial plain radiographs showed gradual resorption CRM; however, difficult identify presence due radiopacity remaining CRM. Histologically, mature lamellar woven osteoblasts osteocytes eight 4 but none 1 2 None Mild-to-moderate infiltration inflammatory around carriers groups. post-operative wound infection found either group. Our results indicate when placed without immunosuppression. determine if a solid fusion can be achieved techniques, further studies needed investigate appropriate dose BMSCs, amounts CRM, requisite incubation time.