Determination of Hepatitis E Virus Seroprevalence by Using Recombinant Fusion Proteins and Synthetic Peptides
作者： D. A. Paul , M. F. Knigge , A. Ritter , R. Gutierrez , T. Pilot-Matias
关键词:
摘要: Recombinant antigens from hepatitis E virus (HEV) open-reading frames 2 and 3 were expressed in Escherichia coli as cytidine monophosphate-2-keto-3-deoxyoctulosonic acid synthetase (CKS) fusion proteins, purified, used to develop an EIA for the detection of antibodies. Serologic results compared with those previous assays by testing 102 samples HEV outbreak Somalia. This CKS/HEV detected anti-HEV all 97 sera found reactive previously additional samples, which shown be true HEV-positive supplemental peptide Western blot tests. The then determine seroprevalence worldwide. ranged 1% 25%, higher rates Middle Eastern countries. Also, 7%-14% acute cases non-A, -B, or -C HEV-positive. Thus, this appears useful detecting various populations.
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