Plant regeneration from protoplasts of Enteromorpha intestinalis (Chlorophyta, Ulvophyceae) as seedstock for macroagal culture
作者： Anne-Marie Rusig , Joël Cosson
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摘要: A continuous micropropagation was established from protoplasts of thegreen alga Enteromorpha intestinalis. The effects two differentcrude enzymes and the osmolarity at different concentrations enzymesolution on algal protoplast yields were tested. optimal enzymecomposition for cell wall digestion viability 2%cellulase R 10 Onozuka 2% Aplysie with 0.5 m mannitol. Largenumbers released (10.0 × 106protoplasts 1 g fresh thalli) settled a rangeof substrata. Regeneration followed normal patternfor this species. Conditions pure cultures efficient systems offloating supports nets determined to optimise product qualityof plantlets Enteromorpha. promising storage process has beendeveloped which involves including in beads alginic acid gel.Plants regenerated may also be used as seedstock tofacilitate propagation macroalgal culture.
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