B-type cyclin modulation in response to carbon balance in callus of Populus alba

摘要: In plants, sucrose is the principal transported carbon compound. Sucrose and/or glucose and fructose are relevant in regulation of meristematic cell proliferation. The aim was to define sugar balance relationship between osmoregulation processes division patterns callus Populus alba response different medium concentrations. Callus proliferation metabolism were assessed through biometric measures, non-structural soluble carbohydrates quantification, osmotic potential determination, as well by quantification mRNA accumulation B-type cyclin genes. Calli cultured on without or supplemented with 10, 20 30 g l−1 sucrose. A large amount used restore medium; upon reaching equilibrium, then for division. initial investment can explain differences lag phase duration decrease concentration. However, calli added both adjust their growth whilst free entered a quiescent state. rates compared transcript trends suggested that threshold effect, rather than quantitative model, governed relation CycB gene transcription findings showed poplar following “hierarchical” model based physiological state concentration available medium.