Characterization of N-myc amplification units in human neuroblastoma cells.

摘要: A set of DNA clones comprising 48 independent HindIII fragments (215 kilobases sequence) was derived from the N-myc amplification unit neuroblastoma cell line NGP. These were used to investigate units in NGP cells and 12 primary tumors. Three parameters evaluated: (i) number rearrangements germ configuration that had occurred during process; (ii) homogeneity within individual tumors; (iii) conservation amplified sequences among different The results indicated remarkably few amplification, any one tumor quite homogeneous, although each contained a unique pattern fragments, there considerable similarity between In particular, strikingly similar over contiguous domain at least 140 surrounding structural gene.