In vitro growth of murine T cells. VI. Accelerated skin graft rejection caused by adoptively transferred cells expanded in T cell growth factor.

摘要: The ability of adoptively transferred cytotoxic T cells expanded in cell growth factor (TCGF) to induce accelerated graft rejection B10.BR skin grafts from normal B6AF1 recipient mice was tested. In vitro sensitized regular TCGF were capable accelerating when i.v. 1 day after grafting. Cells LF-TCGF (lectin-free) more effective than lectin containing TCGF. Nonsensitized or irrelevant B10.D2 antigenic determinants and subsequently unable the grafts. Expanded effector known be immunologically active vivo also accelerate if irradiated with 2000 R before adoptive transfer. lyse appropriate targets 4-hr 51Cr release assays correlated poorly effectiveness these injection. These studies are first demonstrate that mediating a function vivo.