In vitro differentiation of T-cells capable of mediating the regression of established syngeneic tumors in mice.

摘要: Previous studies have shown that successful adoptive immunotherapy of a newly induced, weakly immunogenic murine sarcoma, MCA 105, can be achieved either with fresh noncultured immune spleen cells or after in vitro stimulation and expansion. In this study, we utilized vivo depletions monoclonal antibodies (mAb) T-cell subpopulations expressing the L3T4 Lyt-2 antigens to investigate phenotype T-cells mediate tumor regression. The efficiency depletion was assessed by flow microfluorometric analysis ability specifically treated cell populations generate allogeneic cytotoxic T-lymphocytes. therapeutic efficacy adoptively transferred 105 abrogated administration mAb mice bearing 3-day established pulmonary metastases. treatment complement also their antitumor confirming initial findings. However, mixing complement-treated reconstituted indicating cellular cooperation between these two lymphoid essential for regression tumors. Unlike cells, sensitized expanded but not Lyt-2+ alone played major role mediating These findings provide evidence an -induced differentiation Our results thus suggest activities expressed types may represent at different stages immunological differentiation.