Determination of IAA and ABA in the same plant sample by a widely applicable method using GC-MS with selected ion monitoring
作者： Xiaoyue Li , Clifford E. La Motte , Cecil R. Stewart , Norman P. Cloud , Susan Wear-Bagnall
DOI: 10.1007/BF00193844
关键词:
摘要: A method for the purification and subsequent quantification of indole-3-acetic acid (IAA) abscisic (ABA) from same sample highly pigmented green tissue has been developed tested in several species. Solvent partitioning high-performance liquid chromatography (HPLC) were used purification. Separate fractions HPLC-containing IAA ABA analyzed by gas chromatography-mass spectrometry (GC-MS) using selected-ion monitoring (SIM). Isotope dilution was to correct incomplete recovery. Results are presented samples 11 different species five plant organs. The can be completed, both ABA, two 8 h an experienced technician. dominant peaks chromatograms HPLC-purified samples, amounts about 1 ng detected. extract partitioned into aqueous solution pH 9.5, a step suspected ester hydrolysis. By analyzing known contain esters comparing results with methods which excluded this step, we have shown that does not result erroneously high values due direct comparison one HPLC employed indicates our measures these compounds detectable when is omitted. Thus, essential where contaminating co-purify through solvent-partitioning steps.
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