Analysis of p107-associated proteins: p107 associates with a form of E2F that differs from pRB-associated E2F-1.

摘要: The binding of viral oncogenes to cellular proteins is thought modulate the activities these targets. p107 protein targeted by many proteins, including adenovirus E1A, simian virus 40 large T antigen, and human papillomavirus type 16 E7 protein. A panel monoclonal antibodies against was raised used identify that interact with in vivo. p107-associated included cyclin A, E, cdk2. In addition, found associate 62- 65- 50-kDa phosphoproteins ML-1 cells, a myeloid leukemia cell line. 65-kDa have properties transcription factor E2F but were distinguished from pRB-associated E2F-1 both immunologic biochemical properties.