Ascorbic Acid in Mesencephalic Cultures: Effects on Dopaminergic Neuron Development

摘要: Ascorbic acid exists in high intracellular concentrations fetal rat brain. In mesencephalic cultures the cellular ascorbic content drops sharply to undetectable levels when no is added medium, thus creating a model of scorbutic neuronal tissue and affording study acid's effects on cell development function. Cultures treated with 0.2 mM were compared controls (scorbutic cultures) by using morphological biochemical indices. at 7 14 days vitro showed marked increase glial proliferation fibrillary acidic protein staining increased neurite growth number tyrosine hydroxylase staining. Significantly higher dopamine uptake 3,4-dihydroxyphenylacetic also observed after treatment. The capacity accumulate ability retain developed gradually as matured. reached embryonal day vitro. We conclude that although can survive grow absence detectable acid, its presence exerts broad effect neuron morphology functioning either directly or through proliferation, possibly both.