Pacemaker role of pericytes in generating synchronized spontaneous Ca2+ transients in the myenteric microvasculature of the guinea-pig gastric antrum

摘要: Properties of spontaneous Ca(2+) transients in the myenteric microvasculature guinea-pig stomach were investigated. Specifically, we explored spatio-temporal origin and role voltage-dependent channels (VDCCs) their intercellular synchrony using fluorescence imaging immunohistochemistry. The generated that independent both interstitial cells Cajal (ICC) neural activity. Spontaneous highly synchronous along length microvasculature, appeared to be initiated pericytes spread arteriolar smooth muscle (SMCs). In most cases, generation or was not affected by blockers L-type VDCCs. nifedipine-treated preparations, readily blocked Ni(2+), mibefradil ML216, for T-type These also suppressed known VDCC dependent component ICC slow waves. caffeine, tetracaine cyclopiazonic acid (CPA). After blockade L- VDCCs, asynchronous on precapillary arterioles and/or capillaries but SMCs, abolished CPA nominally free solution. Together these data indicate may act as transients. Pericyte arise from release sarco-endoplasmic reticulum opening VDCCs is required propagation SMCs.