Immune Complex-Mediated Enhancement of Antibody Responses without Induction of Delayed-Type Hypersensitivity

摘要: Immunoglobulin (Ig)G and IgE antibodies enhance the humoral response in vivo to soluble antigens with which they form complexes. In vitro, antigen is targeted B cells by macrophages dendritic (DCs) IgG, thus leading increased presentation specific T cells. Possibly these mechanisms are also responsible for antibody-mediated enhancement vivo. We now address question of whether IgG- and/or IgE-antigen complexes can prime delayed-type hypersensitivity (DTH), a reaction known require primed helper (Th)1 Mice were immunized IgG-anti-2,4,6-trinitrophenyl (TNP)/BSA-TNP or IgE-anti-TNP/BSA-TNP. given BSA-TNP alone complete Freund's adjuvans (CFA) used as controls. DTH IgG-anti-BSA levels measured after subsequent challenge BSA. A potent BSA-specific antibody was induced IgE- IgG-complexed well CFA/antigen but DTH-reactions only observed mice CFA/antigen. Both IgG enhanced production IgG1, IgG2a IgG2b, although most pronounced seen IgG1. These findings suggest that Th2 rather than Th1 involved immune IgE-immune