Differential Association of Tau With Subsets of Microtubules Containing Posttranslationally-Modified Tubulin Variants in Neuroblastoma Cells

摘要: Neuronal cells display different subsets of dynamic microtubules. In axons and extending neurites, this intrinsic dynamics is modulated by the microtubule-associated protein tau. Moreover, posttranslational modifications tubulin, namely acetylation, tyrosination or glutamylation are directly involved in determining stability neuronal Studies were carried out to analyze interaction patterns tau with microtubules N2A neuroblastoma cells, which can differentiate presence dibutyryl cAMP. Double labeling studies showed a differential pattern association containing acetylated tyrosinated tubulin. Furthermore, using depolymerizing drugs revealed selectivity microtubular polymers microfilaments, within organization cytoskeleton. order study specific isoforms modified tubulin variants, immunoprecipitation out. The coimmunoprecipitation data indicated selective binding either variant. To assess hypothesis on roles stabilization tubulins, those variants was analyzed overlay experiments. A preferential from undifferentiated cell extracts, at least one slow-migrating isoform revealed. However, long neurites displayed two On other hand, extracts bound entire set isoforms. These studies, along stability, indicate that segregates into axonal processes. also suggest these interactions may respond functional versatility differentiating neurons.