Evaluation of a generic RT-nested-PCR for detection of flaviviruses in suspected fatal cases of dengue infection, Rio de Janeiro, Brazil.
作者： Josélio Maria Galvão de Araújo , Gabriella Mello Gomes , Nieli Rodrigues da Costa Faria , Eliane Saraiva Machado de Araújo , Ana Maria Bispo de Filippis
DOI: 10.1016/J.JVIROMET.2012.08.010
关键词:
摘要: Flaviviruses are significant causes of disease worldwide and can be classified serologically into several antigenic complexes. The purpose the present study was to evaluate effectiveness a generic RT-nested-PCR for detection flavivirus during dengue outbreak in Brazil 2008. A total 105 serum samples were collected from patients with fatal outcome examined by RT-PCR, conventional IgM serology. RT-PCR confirmed 19 (18%) cases. Conventional performed on detected 45 (42.8%) virus infections. serology 44 102 (43.1%) infecting serotype identified cases (18 DENV-2 1 DENV-3) (40 5 DENV-3). In addition, we analyzed performance RT-PCRs stratified day onset symptoms. Our results indicate that different methods should included surveillance programs, including virological serological approaches.
core.ac.uk
fiocruz.br参考文章(26)
G J Chang, D W Trent, A V Vorndam, E Vergne, R M Kinney, C J Mitchell, An integrated target sequence and signal amplification assay, reverse transcriptase-PCR-enzyme-linked immunosorbent assay, to detect and characterize flaviviruses. Journal of Clinical Microbiology. ,vol. 32, pp. 477- 483 ,(1994) , 10.1128/JCM.32.2.477-483.1994
R S Lanciotti, C H Calisher, D J Gubler, G J Chang, A V Vorndam, Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction. Journal of Clinical Microbiology. ,vol. 30, pp. 545- 551 ,(1992) , 10.1128/JCM.30.3.545-551.1992
Robert S. Lanciotti, Amy J. Kerst, Roger S. Nasci, Marvin S. Godsey, Carl J. Mitchell, Harry M. Savage, Nicholas Komar, Nicholas A. Panella, Becky C. Allen, Kate E. Volpe, Brent S. Davis, John T. Roehrig, Rapid Detection of West Nile Virus from Human Clinical Specimens, Field-Collected Mosquitoes, and Avian Samples by a TaqMan Reverse Transcriptase-PCR Assay Journal of Clinical Microbiology. ,vol. 38, pp. 4066- 4071 ,(2000) , 10.1128/JCM.38.11.4066-4071.2000
V. Pierre, M.-T. Drouet, V. Deubel, Identification of mosquito-borne flavivirus sequences using universal primers and reverse transcription/polymerase chain reaction Research in Virology. ,vol. 145, pp. 93- 104 ,(1994) , 10.1016/S0923-2516(07)80011-2
Luiz Tadeu Moraes Figueiredo, The Brazilian flaviviruses. Microbes and Infection. ,vol. 2, pp. 1643- 1649 ,(2000) , 10.1016/S1286-4579(00)01320-4
Fang Meiyu, Chen Huosheng, Chen Cuihua, Tian Xiaodong, Jiang Lianhua, Peng Yifei, Chen Weijun, Guo Huiyu, Detection of Flaviviruses by Reverse Transcriptase-Polymerase Chain Reaction with the Universal Primer Set Microbiology and Immunology. ,vol. 41, pp. 209- 213 ,(1997) , 10.1111/J.1348-0421.1997.TB01192.X
L. Fulop, A.D.T. Barrett, R. Phillpotts, K. Martin, D. Leslie, R.W. Titball, Rapid identification of flaviviruses based on conserved NS5 gene sequences Journal of Virological Methods. ,vol. 44, pp. 179- 188 ,(1993) , 10.1016/0166-0934(93)90053-T
Hermann G. Schatzmayr, Rita Maria R. Nogueira, Amélia P. A. Travassos da Rosa, An outbreak of dengue virus at Rio de Janeiro - 1986 Memorias Do Instituto Oswaldo Cruz. ,vol. 81, pp. 245- 246 ,(1986) , 10.1590/S0074-02761986000200019
V. T. K. Chow, Catherine L. K. Seah, Y. C. Chan, Use of NS3 consensus primers for the polymerase chain reaction amplification and sequencing of dengue viruses and other flaviviruses. Archives of Virology. ,vol. 133, pp. 157- 170 ,(1993) , 10.1007/BF01309751
Zayd A. Eldadah, David M. Asher, Mark S. Godec, Kitty L. Pomeroy, Lev G. Goldfarb, Stephen M. Feinstone, Herbert Levitan, C. J. Gibbs, D. Carleton Gajdusek, Detection of flaviviruses by reverse-transcriptase polymerase chain reaction. Journal of Medical Virology. ,vol. 33, pp. 260- 267 ,(1991) , 10.1002/JMV.1890330410