Involvement of purinergic system in the release of cytokines by macrophages exposed to glioma‐conditioned medium

摘要: Macrophages are involved in cancer progression. M1 macrophages have an antitumor effect, whereas M2 phenotype associated with tumor growth. The progression of gliomas involves the participation inflammatory microenvironment. Adenosine triphosphate (ATP) can act as pro-inflammatory signal, adenosine has opposite properties. biological effects extracellular nucleotides/nucleosides mediated by purinergic receptors controlled ectonucleotidases. In present work, we evaluated whether glioma-conditioned medium (GL-CM) modulates macrophage differentiation and ATP release pro-and anti-inflammatory cytokines these cells. results show that exposed to GL-CM were modulated M2-like phenotype. HPLC analysis demonstrated presence significant amounts its metabolites. presented decreased AMP hydrolysis increased IL-10 MCP-1 secretion, diminished P1 or P2 antagonists. did not alter IL-6 macrophages, although treatment promoted increase IL-6, which was prevented a P2X7 antagonist. summary, found A2A activation is necessary for IL-10, MCP-1, GL-CM, which, turn, M2-phenotype. study establishes relationship between polarization, cytokine receptor GL-CM. Therefore, data herein contributes advancing field cancer-related inflammation point specific targets modulation glioma-associated macrophages. J. Cell. Biochem. 116: 721–729, 2015. © 2014 Wiley Periodicals, Inc.