Comparative proteomic analysis of male and female venoms from the Cuban scorpion Rhopalurus junceus.
作者： Rodolfo Rodríguez-Ravelo , Cesar VF Batista , Fredy IV Coronas , Fernando Z Zamudio , Lorena Hernández-Orihuela
DOI: 10.1016/J.TOXICON.2015.06.026
关键词:
摘要: Abstract A complete mass spectrometry analysis of venom components from male and female scorpions the species Rhophalurus junceus Cuba is reported. In order 200 individual molecular masses were identified in both venoms, which 63 are identical females genders. It means that a significant difference exists between individuals different sexes, but most abundant present sexes. The relative abundance among Three well defined groups peptides separated identified. first group corresponds to with 1000–2000 Da; second 3500–4500 Da weight, third 6500–8000 Da weights. total 86 rich disulfide bridges found 27 three 59 four bridges. LC-MS/MS allowed identification amino acid sequence determination 31 novel venom. Two new putative K+-channel sequences by Edman degradation. They contain 37 residues, packed assigned systematic numbers: α-KTx 1.18 α-KTx 2.15.
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sci-hub.se 参考文章(21)
Leonardo De Sousa, A. Borges, Aleikar Vásquez-Suárez, Huub JM Op den Camp, Rosa I Chadee-Burgos, Mirna Romero-Bellorín, Jorge Espinoza, Leonardo De Sousa-Insana, Oscar Pino-García, None, Differences in venom toxicity and antigenicity between females and males Tityus nororientalis (Buthidae) scorpions. Journal of venom research. ,vol. 1, pp. 61- 70 ,(2010)
Martha Escalona, Scorpion beta-toxins and voltage-gated sodium channels: interactions and effects. Frontiers in Bioscience. ,vol. 18, pp. 572- ,(2013) , 10.2741/4121
Nahoko Yamaji, Li Dai, Kenji Sugase, Marta Andriantsiferana, Terumi Nakajima, Takashi Iwashita, Solution structure of IsTX. A male scorpion toxin from Opisthacanthus madagascariensis (Ischnuridae). FEBS Journal. ,vol. 271, pp. 3855- 3864 ,(2004) , 10.1111/J.1432-1033.2004.04322.X
Santos Ramírez-Carreto, Verónica Quintero-Hernández, Juana María Jiménez-Vargas, Gerardo Corzo, Lourival D. Possani, Baltazar Becerril, Ernesto Ortiz, Gene cloning and functional characterization of four novel antimicrobial-like peptides from scorpions of the family Vaejovidae. Peptides. ,vol. 34, pp. 290- 295 ,(2012) , 10.1016/J.PEPTIDES.2012.02.002
Ricardo C. Rodríguez de la Vega, Lourival D. Possani, Overview of scorpion toxins specific for Na+ channels and related peptides: biodiversity, structure–function relationships and evolution Toxicon. ,vol. 46, pp. 831- 844 ,(2005) , 10.1016/J.TOXICON.2005.09.006
Xiaobo Xu, Zhigui Duan, Zhiyong Di, Yawen He, Jianglin Li, Zhongjie Li, Chunliang Xie, Xiongzhi Zeng, Zhijian Cao, Yingliang Wu, Songping Liang, Wenxin Li, Proteomic analysis of the venom from the scorpion Mesobuthus martensii. Journal of Proteomics. ,vol. 106, pp. 162- 180 ,(2014) , 10.1016/J.JPROT.2014.04.032
Lourival D. Possani, Baltazar Becerril, Muriel Delepierre, Jan Tytgat, Scorpion toxins specific for Na+-channels. FEBS Journal. ,vol. 264, pp. 287- 300 ,(1999) , 10.1046/J.1432-1327.1999.00625.X
C BATISTA, L DELPOZO, F ZAMUDIO, S CONTRERAS, B BECERRIL, E WANKE, L POSSANI, Proteomics of the venom from the Amazonian scorpion Tityus cambridgei and the role of prolines on mass spectrometry analysis of toxins. Journal of Chromatography B. ,vol. 803, pp. 55- 66 ,(2004) , 10.1016/J.JCHROMB.2003.09.002
Jan Tytgat, K.George Chandy, Maria L Garcia, George A Gutman, Marie-France Martin-Eauclaire, Jurg J van der Walt, Lourival D Possani, A unified nomenclature for short-chain peptides isolated from scorpion venoms: α-KTx molecular subfamilies Trends in Pharmacological Sciences. ,vol. 20, pp. 444- 447 ,(1999) , 10.1016/S0165-6147(99)01398-X
Ricardo C Rodrı́guez de la Vega, Lourival D Possani, Current views on scorpion toxins specific for K+-channels. Toxicon. ,vol. 43, pp. 865- 875 ,(2004) , 10.1016/J.TOXICON.2004.03.022