Differences in venom toxicity and antigenicity between females and males Tityus nororientalis (Buthidae) scorpions.
作者： Leonardo De Sousa , A. Borges , Aleikar Vásquez-Suárez , Huub JM Op den Camp , Rosa I Chadee-Burgos
DOI:
关键词: Immunology 、 Venom Protein 、 Biology 、 Toxicity 、 Physiology 、 Median lethal dose 、 Bradypnea 、 Scorpion 、 Buthidae 、 Venom 、 Antibody
摘要: Venom from male and female specimens of the medically important Venezuelan scorpion Tityus nororientalis have been compared. Males showed a significantly higher venom yield (2.39mg/individual) compared to scorpions (0.98mg/individual). Female was more toxic than that males, with median lethal dose (LD50) in C57BL/6 mice 9.46 μg protein/gm body weight [95% confidence interval (8.91-9.94)] whereas LD50 for males 13.36(12.58-14.03) μg/gm. Mass spectral analyses by MALDI-TOF revealed differences composition between females. From clinical standpoint, time course toxicity indicated tendency, case venom, elicit earlier occurrence severe signs such as sialorrhea, dyspnea (bradypnea/apnea) exophthalmus particularly late phase. less efficient inhibit binding anti-T. discrepans antibodies immobilized T. ELISA assays, suggesting sex-related bioactive surfaces toxins. These results indicate females produce venoms different activity which may epidemiological implications.
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