The in situ inhibition of prothrombinase-formed human alpha-thrombin and meizothrombin(des F1) by antithrombin III and heparin.

作者: P Schoen , T Lindhout

DOI: 10.1016/S0021-9258(18)60954-8

关键词:

摘要: Abstract The inhibition of thrombin by antithrombin III (AT III) and heparin has been studied in pure systems to determine the kinetics during human prothrombin activation. present study shows that prothrombinase-catalyzed activation resulted generation meizothrombin(des F1). In absence second-order rate constants inactivation both F1) formed reaction mixture appeared be identical, k = 3.7 X 10(5) M-1 min-1. constant purified was 6.5 presence decay amidolytic activity biexponential could modeled a four-parameter equation pseudo first-order as well composition with respect levels ratio over varied initial concentration. Heparin catalyzed AT but not Thrombin, generated (Xa-Va-phospholipid-Ca2+) inhibited III/heparin more slowly than thrombin, saturation differed from those found thrombin.

参考文章(26)
R F Zwaal, G Tans, J Rosing, Formation of meizothrombin as intermediate in factor Xa-catalyzed prothrombin activation. Journal of Biological Chemistry. ,vol. 261, pp. 4224- 4228 ,(1986) , 10.1016/S0021-9258(17)35651-X
F J Walker, C T Esmon, The effect of prothrombin fragment 2 on the inhibition of thrombin by antithrombin III. Journal of Biological Chemistry. ,vol. 254, pp. 5618- 5622 ,(1979) , 10.1016/S0021-9258(18)50458-0
K G Mann, M Nesheim, M N Blackburn, C M Lawler, Dependence of antithrombin III and thrombin binding stoichiometries and catalytic activity on the molecular weight of affinity-purified heparin. Journal of Biological Chemistry. ,vol. 261, pp. 3214- 3221 ,(1986) , 10.1016/S0021-9258(17)35770-8
C H Pletcher, G L Nelsestuen, The rate-determining step of the heparin-catalyzed antithrombin/thrombin reaction is independent of thrombin. Journal of Biological Chemistry. ,vol. 257, pp. 5342- 5345 ,(1982) , 10.1016/S0021-9258(19)83781-X