Pools of lipidated HTL-CTL constructs prime for multiple HBV and HCV CTL epitope responses

摘要: Various peptide-based approaches to simultaneous induction of multiple cytotoxic T lymphocyte (CTL) responses were evaluated as part ongoing efforts develop immunotherapeutic vaccines for use in humans. To this end, HLA (human histocompatibility leukocyte antigen)-A2-restricted epitopes from several specific viral proteins tested an HLA-A2 transgenic mouse model system, which mimics human CTL these proteins. Multiple elicited by immunization with either peptides emulsified incomplete Freund's adjuvant (IFA), or lipidated administered phosphate buffered saline (PBS). In the case peptides, was crucially dependent on presence helper (HTL) epitopes, and most efficient covalently linked HTL-CTL epitope constructs. could also be induced HTL simply mixed formulated PBS. However, approach highly particular HTL/CTL combination utilized, marginally effective priming responses. By contrast, all combinations potent immunogens when delivered lipidated, molecules. This analysed terms multi-epitope priming, demonstrated a pool five different epitopes.