Lymphocyte function in human bone marrow. I. Characterization of two T cell populations regulating immunoglobulin secretion.

摘要: We analyzed the regulation of immunoglobulin (Ig) production in short-term cultures human (rib) bone marrow cells. In contrast to blood or tonsil cell cultures, large quantities IgG and IgA, but not IgM, were secreted by unstimulated The addition pokeweed mitogen phytohemagglutinin resulted suppression this Ig secretion. Both mitogens induced high levels interleukin 2 (IL 2) IL alone mimicked suppressive effect mitogens. Incubation cells with Epstein Barr virus enhanced secretion, primarily IgM isotype. suppressed these as well. mitogen-induced secretion stimulated was inhibited monoclonal anti-TAC receptor) antibody. Cell separation experiments indicated that induction suppressor activity involved two distinct populations marrow-resident T lineage first population responds activation 2. This has a surface phenotype appropriate for helper second expresses T8 TAC determinants. These acquire after exposure expression function does require additional (e.g., mitogenic) signals. 2-dependent represent newly recognized lymphocyte subset. regulatory pathway delineated may be important antibody formation marrow, major site man.