Limiting dilution analysis of the B cell compartment in human bone marrow.
作者： Toshifumi Hibi , Hans-Michael Dosch
关键词: Bone marrow 、 Population 、 Biology 、 Immunology 、 Epstein–Barr virus 、 B-1 cell 、 B cell 、 Antigen 、 Antibody 、 Molecular biology 、 Immunoglobulin E
摘要: Mononuclear cells (MNC) obtained from adult rib specimen were investigated for their capacity to produce immunoglobulin (Ig) in vitro. Using limiting dilution analysis 3 populations of B lineage could be distinguished. The first produces Ig culture without any intentional activation. These are strictly nonproliferating and sustain extraordinary secretory rates 5 X 10(7)-10 10(7) molecules IgG, IgA or IgM/cell/h at least 2 weeks. They occur frequencies 1 10(-4)-10 10(-4) (marrow MNC) represent 1-5% marrow cells. Following exposure infectious Epstein-Barr virus (EBV), these do not (a) proliferate, (b) express EBV-determined nuclear antigens (EBNA), (c) enhance (d) show activity prolonged time. therefore EBV resistant. If produced antibody similar vivo, then would suggest that they provide up 2/3 daily synthetic IgG 20-30% the IgM production. second population is exclusively committed EBNA begin secrete IgM. third represents 90% In our hands, unable
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