Daily application of low magnitude mechanical stimulus inhibits the growth of MDA-MB-231 breast cancer cells in vitro

摘要: Mechanical loads can regulate cell proliferation and differentiation at various stages of development homeostasis. However, the extension this regulatory effect mechanical on cancer cells is largely unknown. Increased physical compliance one key features cells, which may hamper transmission to these within tumor microenvironment. Here we tested whether brief daily application an external low magnitude stimulus (LMMS), would impede growth MDA-MB-231 aggressive type breast in vitro for 3 wks growth. The signal was applied oscillatory form 90 Hz 0.15 g, a regimen that induce via inertial properties rather than matrix deformations. Experimental were exposed LMMS 15 min/day, 5 days/week ambient conditions while control sham loaded. Cell proliferation, viability, cycle, apoptosis, morphology migration Trypan Blue dye exclusion, MTT, PI, Annexin V, Calcein-AM phalloidin stains scratch wound assays. Compared controls, reduced number viability cancerous significantly after first week culture, non-cancerous MCF10A found be unaffected. Flow cytomety analyses suggested observed decrease group due cycle arrest apoptosis. further circularity increased cytoskeletal actin cells. Combined, results suggest direct negatively If confirmed, non-invasive approach integrated efforts prevention and/or treatment cancer.