Analysis of bilirubin and bilirubin mono- and di-conjugates. Determination of their relative amounts in biological samples.
作者： N Blanckaert
DOI: 10.1042/BJ1850115
关键词:
摘要: 1. A novel method for determination of the relative amounts unconjugated bilirubin and sugar mono- di-conjugates in biological samples, including serum, is described illustrated by its application to analysis bilinoids rat bile. 2. The based on specific conversion carbohydrate conjugates into corresponding or di-methyl esters base-catalysed transesterification methanol. Under selected reaction conditions, biliru-in remains intact no dipyrrole exchange detectable; di-glucuronide virtually complete (approx. 99%), sponification negligible (less than 1%); recovery pigments approx. 95%. 3. products methyl are separated t.l.c. determined spectrophotometrically; two isomeric bilirubin-IX alpha monomethyl therefore can be individually. 4. Reference have been synthesized characterized, isomers ester dimethyl were obtained individually, crystalline form. 5. With this new method, all (over 99%) normal bile found conjugated, with diconjugates (71%) predominating. significantly increased proportion monoconjugates present collected from heterozygous Gunn rats that refused large bilirubin.
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