A Baculovirus-Encoded MicroRNA (miRNA) Suppresses Its Host miRNA Biogenesis by Regulating the Exportin-5 Cofactor Ran

摘要: MicroRNAs have emerged as key players in the regulation of various biological processes eukaryotes, including host-pathogen interactions. Recent studies suggest that viruses encode miRNAs to manipulate their host gene expression ensure effective proliferation, whereas limits virus infection by differentially expressing target essential viral genes. Here, we demonstrate an insect virus, Bombyx mori nucleopolyhedrosis (BmNPV), modulates small-RNA-mediated defense its host, B. mori, encoding miRNA (bmnpv-miR-1) downregulates GTP-binding nuclear protein Ran, component exportin-5-mediated nucleocytoplasmic transport machinery mainly involved small-RNA from nucleus cytoplasm. We sequence-dependent interaction bmnpv-miR-1 with Ran mRNA using cell culture and vivo assays, RNA interference (RNAi) Ran. Our results clearly show represses leading reduction population, consequently, BmNPV load increases infected larvae. Blocking resulted higher levels a decrease proliferation. In contrast, blockage miRNA, bmo-miR-8, which targets immediate-early whose production was repressed upon dsRNA administration, significant increase The present study provides insight into one evasion strategies used counter for proliferation has relevance development control strategies.