Recombinant Candida utilis for the production of biotin.

摘要: Biotin is an important nutritional supplement but difficult to manufacture effectively. Here we present a trial of biotin production using the food yeast Candida utilis. In this system, cloned C. utilis synthase (BIO2) gene, gene rate-limiting enzyme for biosynthesis, and assembled it under control strong promoter. A series plasmids were constructed direct integration BIO2 either high-copy with 18S rDNA fragment or low-copy URA3 HIS3 fragment. The can be successfully integrated into chromosome drive these plasmids. yield in system reach 100-fold above endogenous level small-scale culture. Although not stable if selection pressure removed, has potential produce biotin-rich feed additives directly without requirement further purification.