Recombinant Rhizobium meliloti strains with extra biotin synthesis capability.
作者： W R Streit , D A Phillips
DOI: 10.1128/AEM.62.9.3333-3338.1996
关键词: Microbiology 、 Rhizobium 、 Rhizosphere 、 Sinorhizobium meliloti 、 Rhizobiaceae 、 Biochemistry 、 Bacteria 、 Biology 、 Biotin 、 Auxotrophy 、 Escherichia coli
摘要: The growth of Rhizobium meliloti 1021 in an experimental alfalfa (Medicago sativa L.) rhizosphere was stimulated by adding nanomolar amounts biotin. To overcome this biotin limitation, R. strains were constructed conjugating the Escherichia coli synthesis operon into auxotroph 1021-B3. Transconjugant Rm1021-WS10 and Rm1021-WS11 grew faster vitro achieved a higher cell density than did overproduced on defined medium. increase yield associated with as much 99% loss viability for Rm1021-WS11, but data suggested that separate stabilizing factor E. DNA reduced death Rm1021-WS10. In tests, recombinant showed delayed competed poorly against Rm1021.
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