Optimization and evaluation of Flexicult® Vet for detection, identification and antimicrobial susceptibility testing of bacterial uropathogens in small animal veterinary practice

摘要: Urinary tract infection (UTI) is a common reason for antimicrobial prescription in dogs and cats. The objective of this study was to optimize evaluate culture-based point-of-care test detection, identification susceptibility testing bacterial uro-pathogens veterinary practice. Seventy-two urine samples from cats with suspected UTI presenting seven facilities were used by clinical staff an investigator estimate sensitivity specificity Flexicult Vet A compared laboratory reference standards culture testing. Subsequently, the modified inclusion oxacillin-containing compartment detection methicillin-resistant staphylococci. performance product (Flexicult B) evaluated vitro using collection 110 isolates. Bacteriuria reported 25 (35 %) field study. bacteriuria 83 100 %, respectively. Bacterial species correctly identified 53 100 % positive investigator, results interpreted 70 % 94 drug-strain combinations. Higher percentages correct interpretation observed when both (76 %) (94 %). most frequent errors false resistance β-lactams (ampicillin, amoxicillin-clavulanate cephalotin) Escherichia coli A, E. ampicillin Staphylococcus pseudintermedius B. latter error can be prevented categorizing staphylococcal strains growing oxacillin as resistant all β-lactams. Despite shortcomings regarding β-lactam coli, B (commercial name Flexicult® Vet) time- cost-effective guide choice facilitate implementation use guidelines treatment UTIs small animals, provided that adequately trained interpret clinics meet minimum operate in-house culture.