RAPD Assessment for Identification of Clonal Identity and Genetic Stability of in vitro Propagated Chestnut Hybrids

摘要: Randomly amplified polymorphic DNA (RAPD) was used as a tool to assess the clonal identity of four in vitro propagated chestnut rootstock hybrids (Castanea sativa × C. crenata) described originally isolated from same mother tree. To confirm genetic stability after multiplication for more than 4 years, RAPD patterns and donor plants were compared. From 40 arbitrary 10-mer primers amplify DNA, 21 provided chosen comparisons. Although significant differences found growth parameters between material putative clones, profiling showed polymorphism none but one. This accession may then be withdrawn origin other three. As expected, no detected they originated from.