A comparison of DNA methylation specific droplet digital PCR (ddPCR) and real time qPCR with flow cytometry in characterizing human T cells in peripheral blood
作者： John K Wiencke , Paige M Bracci , George Hsuang , Shichun Zheng , Helen Hansen
DOI: 10.4161/15592294.2014.967589
关键词:
摘要: Quantitating the copy number of demethylated CpG promoter sites of the CD3Z gene can be used to estimate the numbers and proportions of T cells in human blood and tissue. …
sci-hub.se 参考文章(37)
Ian F. Parney, Basic Concepts in Glioma Immunology Advances in Experimental Medicine and Biology. ,vol. 746, pp. 42- 52 ,(2012) , 10.1007/978-1-4614-3146-6_4
Cleo E. Rolle, Sadhak Sengupta, Maciej S. Lesniak, Mechanisms of immune evasion by gliomas. Advances in Experimental Medicine and Biology. ,vol. 746, pp. 53- 76 ,(2012) , 10.1007/978-1-4614-3146-6_5
M J Brisco, A A Morley, P J Sykes, E Hughes, J Condon, S H Neoh, Quantitation of targets for PCR by use of limiting dilution. BioTechniques. ,vol. 13, pp. 444- 449 ,(1992)
Stephen A Bustin, Tania Nolan, Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction. Journal of biomolecular techniques. ,vol. 15, pp. 155- 166 ,(2004)
J.CIark Susan, Janet Harrison, Cheryl L. Paul, Marianne Frommer, High sensitivity mapping of methylated cytosines. Nucleic Acids Research. ,vol. 22, pp. 2990- 2997 ,(1994) , 10.1093/NAR/22.15.2990
Konstantinos Gousias, Alexander von Ruecker, Paraskevi Voulgari, Matthias Simon, Phenotypical analysis, relation to malignancy and prognostic relevance of ICOS+T regulatory and dendritic cells in patients with gliomas. Journal of Neuroimmunology. ,vol. 264, pp. 84- 90 ,(2013) , 10.1016/J.JNEUROIM.2013.09.001
Udo Baron, Stefan Floess, Georg Wieczorek, Katrin Baumann, Andreas Grützkau, Jun Dong, Andreas Thiel, Tina J. Boeld, Petra Hoffmann, Matthias Edinger, Ivana Türbachova, Alf Hamann, Sven Olek, Jochen Huehn, DNA Demethylation in the Human FOXP3 Locus Discriminates Regulatory T Cells From Activated FOXP3(+) Conventional T Cells European Journal of Immunology. ,vol. 37, pp. 2378- 2389 ,(2007) , 10.1002/EJI.200737594
Maja Kiselinova, Alexander O. Pasternak, Ward De Spiegelaere, Dirk Vogelaers, Ben Berkhout, Linos Vandekerckhove, Comparison of droplet digital PCR and seminested real-time PCR for quantification of cell-associated HIV-1 RNA. PLOS ONE. ,vol. 9, ,(2014) , 10.1371/JOURNAL.PONE.0085999
N. Cézé, G. Thibault, G. Goujon, J. Viguier, H. Watier, E. Dorval, T. Lecomte, Pre-treatment lymphopenia as a prognostic biomarker in colorectal cancer patients receiving chemotherapy. Cancer Chemotherapy and Pharmacology. ,vol. 68, pp. 1305- 1313 ,(2011) , 10.1007/S00280-011-1610-3
Olivier Trédan, Manuarii Manuel, Gilles Clapisson, Thomas Bachelot, Sylvie Chabaud, Christine Bardin-dit-Courageot, Chantal Rigal, Cathy Biota, Agathe Bajard, Nicolas Pasqual, Jean-Yves Blay, Christophe Caux, Christine Ménétrier-Caux, Patients with metastatic breast cancer leading to CD4+ T cell lymphopaenia have poor outcome European Journal of Cancer. ,vol. 49, pp. 1673- 1682 ,(2013) , 10.1016/J.EJCA.2012.11.028