Establishment and Characterization of an Omasal Epithelial Cell Model Derived from Dairy Calves for the Study of Small Peptide Absorption

摘要: The objective of this study was to establish a primary culture omasal epithelial cells (OECs) derived from dairy calves and characterize its function in small peptide absorption. Bovine tissues were obtained newborn Chinese Holstein digested with 2.5% trypsin solution obtain OECs. isolated later cultured DMEM containing 10% fetal bovine serum, 5 μg/ml insulin, 10 ng/ml epidermal growth factor, 100 U/ml penicillin, streptomycin, 50 gentamycin 2.5 amphotericin B. Hematoxylin eosin staining tissue after digestion indicated that the originated strata. Pure displayed an cell-like morphology, similar cobblestone, few visible fibroblasts cytokeratin 18-positive according immunocytochemical analyses. OECs morphologically characterized desmosomes, tight junctions microvilli. These exhibited normal properties, as assessed using cell curve, stably for passages. expressed transporter 1 (PEPT1) mRNA absorbed intact glycylsarcosine (Gly-Sar). uptake Gly-Sar by pH-dependent optimal pH 5.5–6.5. Furthermore, also time-dependent, concentration-dependent temperature-dependent. Moreover, PEPT1 saturated at concentration mM. via higher compared passive route low substrate concentrations (<1.5 mM). This result suggested contributed more total absorption concentrations. In addition, could be competitively inhibited methionine-glycine. Taken together, these data contributes Thus, may serve useful model peptides omasum.