Substrate specificity of cis-prenyltransferase in rat liver microsomes.
作者： J Ericsson , A Thelin , T Chojnacki , G Dallner
DOI: 10.1016/S0021-9258(18)41836-4
关键词:
摘要: Long chain cis-prenyltransferase in rat liver microsomes was studied using various allylic isoprenoid substrates. Microsomes could utilize trans-geranyl pyrophosphate, but not cis-geranyl pyrophosphate for polyprenyl synthesis. Both trans, trans-farnesyl and trans,cis-farnesyl were used as substrates with Km values of 24 5 microM, respectively. trans,trans,cis-Geranylgeranyl be substrate an apparent 36 microM. trans,trans,trans-Geranylgeranyl also utilized substrate, a very low affinity. After pulse labeling 4 min, [3H]isopentenyl trans,trans-farnesyl the only product formed trans,trans,cis-geranylgeranyl which, upon chasing, yielded pyrophosphate. Independent nature used, even case 12-pyrophosphate all-trans-nonaprenyl lengths products identical, i.e. pyrophosphates 15-18 isoprene residues. able to synthesize substrate. The results indicate that contain farnesyl synthase activity reaction catalyzed by may consist two individual steps, synthesis elongation this long pyrophosphates.
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