Analysis of Mopc‐315 Plasmacytoma By Elutriation and Flow Cytometry
作者： Dagmar Flentje , Michael Flentje , Fred Valeriote , Stephen Sapareto
DOI: 10.1111/J.1365-2184.1984.TB00579.X
关键词:
摘要: Intravenously transplanted murine plasmacytoma MOPC-315 cells were separated from normal spleen a tumour-bearing mouse by elutriation and characterized according to morphology, immunologic properties clonogenicity. Morphologically, both lymphocytoid plasmacytoid separable elutriation. Flow cytometry correlated DNA content intracytoplasmic IgA demonstrated two distinct populations, in cell cycle, but with markedly different cellular levels. Density gradient separation the lower-density lower higher From these studies model of differentiation is proposed.
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