Myeloma Growth Kinetics: Potential Applications of Flow Cytometry

摘要: Publisher Summary This chapter discusses myeloma growth kinetics and presents potential applications of flow cytometry (FCM). Myeloma cells can be identified by their surface intracytoplasmic monoclonal immunoglobulins, abnormal DNA content, potentially they could also cytometric measurement RNA or protein, either as a single parameter perhaps more effectively simultaneous dual measurement. FCM has provided way to quantify other cellular components such immunoglobulin receptors using fluoresceinated antibodies. are unique in that have membrane immunoglobulins. They often aneuploid and, therefore, may contents. protein content frequently abnormal. enables the any combination these characteristics made simultaneously on each cell at rates up 5,000 per second. Thus, multiparameter analysis systems particularly suitable for study multiple myeloma. Specific include detection aneuploidy; proliferative changes after drug perturbation; amounts immunoglobulin, RNA, total before perturbation.