Xenograft rejection of porcine islet-like cell clusters in normal, interferon-gamma, and interferon-gamma receptor deficient mice.

摘要: BACKGROUND The aim of the present study was to evaluate role T-cell cytokine interferon (IFN)-gamma in mediating macrophage activation xenograft rejection. METHODS For this purpose, fetal porcine islet-like cell cluster (ICC) transplants were placed under renal capsule normal mice and with a homozygous targeted disruption IFN-gamma or receptor gene. Some continuously infused cyclosporine (CsA, 12.4 mg/kg body weight/day) CsA vehicle by subcutaneously implanted osmotic pumps. Histological evaluation xenografts performed 6 12 days after transplantation. RESULTS All animals, irrespective recipient group, readily rejected ICC xenograft, although rejection process slightly delayed deficient IFN-gamma. Analysis infiltrating cells within knockout revealed pattern similar that found control animals. Six transplantation, there an abundant infiltration macrophages (Mac-1, F4/80, major histocompatibility complex II markers) grafts. Quite contrast, only low moderate number T (CD3 marker) Treatment had no effect on process. In grafts removed from gene, occasional surviving endocrine cells, some cases also few intact ICC, otherwise obliterated xenograft. Few obtained other groups mice. CONCLUSIONS Thus, demonstrates activation, subsequent destruction can operate absence pig-to-mouse model.