A rapid and simple method for the esterification of fatty acids and steroid carboxylic acids prior to gas-liquid chromatography.

摘要: Abstract A simple procedure for the esterification of steroid carboxylic acid groups using an acetyl chloride/alcohol reagent has been carefully evaluated. The ease with which these esters can be formed this depends on and ester required. C24 bile acids form methyl chloride/methanol (1:20, v/v) almost instantaneously at 50°C, whereas C20 aetienic studied requires 2 h 50°C. formation isopropyl 5β-cholanic takes longer (4 50°C) than n-propyi (1 50°C). identity various cholanic have checked by gas chromatography-mass spectrometry other procedures used methylation fatty examined to establish whether they quantitative acids.