The estimation of vitamin D and some metabolites in human plasma by mass fragmentography
作者： D.A. Seamark , D.J.H. Trafford , H.L.J. Makin
DOI: 10.1016/0009-8981(80)90374-5
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摘要: Abstract A mass fragmentographic assay for vitamin D 3 , 25-hydroxyvitamin 2 and 24,25-dihydroxyvitamin is described. After extraction of plasma with methanol dichloromethane, Lipidex 5000 chromatography was used to separate the extract into three fractions (a fraction, a fraction fraction). Cholesterol removed from by thin-layer chromatography. addition each isotachysterol trimethylsilyl ethers were formed. Recoveries prior gas chromatography-mass spectrometry assessed use tritiated standards added before extraction. Using this procedure, normal ranges established (vitamin : 3–17.7, 25-hydroxyvitaminD 5.9–35.2, 0.6–1.0 0.6–2.9 μg/l).
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