Lipopolysaccharide alters decorin and biglycan synthesis in rat alveolar bone osteoblasts: consequences for bone repair during periodontal disease.

摘要: A prime pathogenic agent associated with periodontitis is lipopolysaccharide (LPS) derived from Porphyromonas gingivalis. This study investigated the effects of P. gingivalis LPS on osteoblasts, which are responsible for alveolar bone repair. Bone cells were obtained explants rat chips and cultured 0-200 ng ml(-1) LPS. significantly increased cell proliferation inhibited osteoblast differentiation, as judged by reduced alkaline phosphatase activity. Analysis biglycan mRNA protein levels indicated that delayed normally high expression during early stages culture, differentiation progenitor cells. In presence LPS, decorin was periods culture relating to collagen fibrillogenesis mineral deposition. glycosaminoglycan chains conjugated these proteoglycans suggested in dermatan sulfate persisted within matrix. suggests influences processing matrix, altering activity phenotype development. The consequences this altered relation hindering repair part cycle events periodontal disease discussed.