Characterization of epidermal growth factor receptors on plasma membranes isolated from rat gastric mucosa.

摘要: The binding of human epidermal growth factor (hEGF), β-urogastrone, to plasma membranes isolated from rat gastric mucosa was studied characterize EGF receptors. [125I]hEGF temperature dependent, reversible, and saturable. A single class sites for with a dissociation constant 0.42 nM maximal capacity 42 fmol/mg protein suggested. There little change in the upon addition peptide hormones (secretin, insulin), antiulcer drugs (cimetidine), or an ulcer-inducing reagent (aspirin). Cross-linking use disuccinimidyl suberate resulted labeling 150 kDa. These results indicate presence receptors on mucosa.