Epidermal growth factor

摘要: Abstract Epidermal growth factor, a 6,400-dalton polypeptide from the mouse submaxillary gland, binds specifically to cells and membranes derived variety of human, rat, mouse, bovine tissues. Liver, placenta, skin, cornea, cultured chondrocytes, Hela cells, Chang liver bind large amounts epidermal whereas fat resting lectin-stimulated human peripheral lymphocytes, thymocytes, rat hepatoma mammary virgin pregnant mice little or no factor. The binding site for factor is distinct receptors other anabolic peptides such as insulin, nerve hormone. rapid reversible. rate constant association approximately 10 6 mole −1 sec , dissociation about × −4 apparent equilibrium −9 m . Trypsin at low concentrations (50–200 μg/ml) destroys receptor by not accompanied appreciable degradation peptide present in medium that bound membranes. Use can be made high affinity specificity measure competitive assay 200 pg EGF per ml (3 −11 ).